Casman Medium Base is used with blood for the isolation of Haemophilus influenzae, Neisseria gonorrhoeae and other fastidious microorganisms in a laboratory setting. Casman Medium Base is not intended for use in the diagnosis of disease or other conditions in humans.
In 1947, Casman described an infusion-free medium enriched with 5% blood for fastidious microorganisms incubated anaerobically. This medium replaced formulas containing fresh meat infusion and heated blood. Casman adjusted the formula after experiments revealed nicotinamide disrupted the action of a blood enzyme that inactivates V factor (NAD). The concentration of nicotinamide was lowered to support growth of Neisseria spp.
- Analyte: Haemophilus influenzae, Neisseria gonorrhoeae
- Platform: dehydrated culture media
- Formula: enzymatic digest of casein 5.0g/L, enzymatic digest of animal tissue 5.0g/L, yeast extract 10.0g/L, beef extract 3.0g/L, niacinamide 0.05g/L, p-aminobenzoic acid 0.05g/L, dextrose 0.5g/L, corn starch 1.0g/L, sodium chloride 5.0g/L, agar 13.5g/L
- Grams per litre: 43.00
- Litres per 500g: 12.00
- Plates per 500g: 630.00
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